A Laboratory Manual of Analytical Methods of Protein Chemistry, Volume 5 presents the laboratory techniques for protein and polypeptide study. This book discusses the staining procedure for histones, which has a high degree of selectivity for basic proteins and the unique ability to visualize qualitative differences in terms of color changes. Organized into four chapters, this volume begins with an overview of the formalin-mediated ammoniacal-silver staining procedure as a selective stain for basic proteins and its application per cell and per extract. This text then examines the optical rotatory dispersion (ORD), which has advanced into a powerful tool for describing the conformations and conformational changes of biopolymers. Other chapters consider the application of ultrasensitive calorimetry to thermodynamic problems. This book discusses as well the principle of the technique, its instrumentation, and experimental procedures. The final chapter deals with the hydrodynamic densities and preferential hydration values for protein precipitates in concentrated salt solutions. This book is a valuable resource for chemists and biochemists.P. Alexander, H. P. Lundgren. and to Professors Peter J. W. Debye and Marvin Johnson, and Drs. Koloman Laki and Robert F. Steiner for their most valuable comments. ... 10. O. WARBURG, Stoffwechsel der Tumoren, Berlin, 1926. 11. H. HARNED and B. Owen, The Physical Chemistry of Electrolytic Solutions, Reinhold, New York, 1943 ... Proceedings of the National Academy of Sciences 52, 980 (1964).
|Title||:||A Laboratory Manual of Analytical Methods of Protein Chemistry|
|Author||:||P. Alexander, H. P. Lundgren|
|Publisher||:||Elsevier - 2014-05-17|