Why a Second Edition? The Second Edition provides practical answers to the general question, qHow can I obtain useful sequence information from my protein or peptide?q rather than the more specific question asked in the first edition, qHow can I obtain the N-terminal sequence?q Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. Key Features Highlights of the Second Edition: * Mass spectrophotometric characterization of proteins and peptides * N-terminal sequencing of proteins with blocked N termini * Internal amino acid sequence analysis after protease digestion in-gel and on-blot * Improved microscale peptide purification methods * Computer analysis of protein sequences * New protocols tested and refined through everyday use in authors' laboratories * Updated reference chapter covering all aspects of protein microsequencingWith 50a1000 ug of material, the reverse phase, ion exchange, and gel filtration modes of HPLC and FPLC have proven to be the methods of choice for achieving final purification because of their speed and resolving power. FPLC columnsanbsp;...
|Title||:||A Practical Guide to Protein and Peptide Purification for Microsequencing|
|Author||:||Paul T. Matsudaira|
|Publisher||:||Elsevier - 2012-12-02|