'Advanced Techniques in Soil Microbiology' presents a wide range of biotechnological methods for application in soil microbiology analysis. These include all essential methods involving molecular biology, immunology, microbiology, and structural biology, such as transcriptome analysis, RNAi technology, molecular matchmaking, RAPD, T-RFLP and FT/MS. The techniques and procedures have been selected with the aim of offering practical guides for immediate use in the laboratory. The systems investigated range from individual molecules and cells to entire eukaryotic organisms, with a focus on bacteria, fungi, mycorrhiza, and higher plants. This volume of state-of-the-art, practice oriented methods will be of great use both to the first-timer and to the experienced scientist.Grind the freeze-dried mycelia (5g) using liquid nitrogen and transfer the powdered mycelium into Eppendorf tubes (2 ml). ... chloroform isoamyl alcohol and transfer the final aqueous phase to a new Eppendorf tube using a large bore pipette.
|Title||:||Advanced Techniques in Soil Microbiology|
|Author||:||Ajit Varma, Ralf Oelmüller|
|Publisher||:||Springer Science & Business Media - 2007-07-25|