Antibodies are indispensable tools for research, diagnosis, and therapy. Recombinant approaches allow the modification and improvement of nearly all antibody properties, such as affinity, valency, specificity, stability, serum half-life, effector functions, and immunogenicity. qAntibody Engineeringq provides a comprehensive toolbox covering the well-established basics but also many exciting new techniques. The protocols reflect the latest qhands onq knowledge of key laboratories in this still fast-moving field. Newcomers will benefit from the proven step-by-step protocols, which include helpful practical advice; experienced antibody engineers will appreciate the new ideas and approaches. The book is an invaluable resource for all those engaged in antibody research and development.Roland E. Kontermann, Stefan DA¼bel ... Centrifuge the crude extract in order to separate insoluble cell debris from soluble protein (20, 000 g, 30 min at 4 C). ... over both columns can be done within only 30 min either by manual operation or by running a program automatically. ... 27.3 Tubing diagram for rapid two-column purification of antibody 356 J.V. Schaefer and A. Plu Ickthun Purification of scFv anbsp;...
|Author||:||Roland E. Kontermann, Stefan Dübel|
|Publisher||:||Springer Science & Business Media - 2010-03-10|