The goal of C. elegans is to provide overviews and detailed step-by-step descriptions of newer and state-of-the-art methods utilized in the field. These include tools essential for forward and reverse genetic analysis, data mining, and comparative genomics strategies, electron and fluorescence microscopy methods, automated imaging methods for worm behavioral analysis, functional genomics strategies, and methods for physiological analyses including somatic cell culture, toxicity assays, electrophysiology, and in vivo imaging of intracellular Ca2+ and pH using genetically encoded fluorescent indicator proteins.When 80% of the eggshells have been lysed, add 800 L of L-15 cell culture medium to the Eppendorf tube. Pellet the eggs ... Gently dissociate the cells by repeatedly pipetting the cell suspension using a 1 mL Eppendorf pipettor. Monitor theanbsp;...
|Publisher||:||Springer Science & Business Media - 2006-01-01|