Containing a comprehensive collection of convenient and quantitative methods for studying centrosomes, spindle pole bodies and related organelles, this text is a valuable resource for researchers and others interested in studying the role of these organelles in cell replication. Chapters outlining the role of these organelles in other cell functions are also included, and a wide variety of experimental systems for analyzing these organelles are presented. Detailed protocols for experiments are contained in each chapter for researchers to perform in their own labs. This volume outlines key methodologies used to analyze centrosomes and spindle pole bodies, their replication, and reproduction in the clear, well-illustrated style of the METHODS IN CELL BIOLOGY series.At this stage, use a polypropylene Pasteur pipette with 2 cm cut off the tip to gently remove debris, puffballs, and so on ... After this second spin, remove the cytoplasm from the top of the Eppendorf tube with a clean 20-gauge needle/ syringeanbsp;...
|Title||:||Centrosomes and Spindle Pole Bodies|
|Publisher||:||Gulf Professional Publishing - 2001|