The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM. Covers many areas of CLEM by the best international scientists in the field Includes a brief history and overview on CLEM methods(2000). Correlative light-electron microscopy reveals the tubular-saccular ultrastructure of carriers operating between ... In A. Cavalier, D. Spehner, aamp; B. M. Humbel (Eds.), Hand book of cryo-preparation methods for electron microscopy ( pp.
|Title||:||Correlative Light and Electron MIcroscopy|
|Publisher||:||Academic Press - 2012-10-23|