Cytoskeletal Mechanisms During Animal Development

Cytoskeletal Mechanisms During Animal Development

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The cytoskeleton is central to cell biology and is instrumental in developmental events. Volume 31 is divided into two major sections: nonchordate development and chordate development. Summaries of each section compare evolutionary conservation among organisms.To identify actin filaments, isolated cortices on grids are incubated with 5 mg/ml heavy meromyosin (prepared from ... use of Murraya#39;s clear (a mixture of one part benzyl alcohol : two parts bezyl benzoate) have solved these problems. Eggs are rinsed briefly in the AS solution and fixed in the AS solution containing 3.5% formaldehyde and 0.9% Triton X-100 for 60 min. ... B-tubulin (1 :2000 in PBS plus 5% BSA and 0.1% sodium azide) for 24 hr at 4AdC, followed by rinsing in six changes ofanbsp;...

Title:Cytoskeletal Mechanisms During Animal Development
Publisher:Academic Press - 1996-02-09


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