Appropriate material for cementum scaffolds are selected, and a process to manufacture well-defined scaffolds to regenerate cementum is developed. The well-studied Laminated Object Manufacturing (LOM) process is modified for the manufacture of cementum scaffolds. The scaffolds made with poly(lactic-co-glycolic acid) (PLGA) and bacterial cellulose layers were compared to salt-leach scaffolds made of PLGA. Material properties of four types of scaffolds are tested using ASTM standards and compared to native cementum. Mesenchymal stem cells (MSC) were seeded in the scaffolds and the viability was tested using Alamar Blue assays. Scanning Electrom Microscopy (SEM) was used to evaluate the surface morphology, texture and cell growth. Calcium deposits were also tested. Results from theses studies show that the scaffolds generated by these methods are possible precursors for further testing and generating cementum in vivo.the tooth with a focus on cementum formation is examined. The physical, mechanical, and biochemical properties of cementum are reviewed in order to understand and develop a new scaffold for cementum that uses tissue engineeringanbsp;...
|Title||:||Development of Well-defined Periodontal Scaffolds Consisting of Poly(lactic-co-glycolic Acid) and Bacteria Cellulose|
|Publisher||:||ProQuest - 2007|