The process of developing nucleic acid probes for infectious agents and novel methods of detecting the binding of probes to the target DNA and RNA are reviewed in this thorough volume. Probes to a wide variety of bacterial, viral and protozoan pathogens are presented in detail by the leaders of this dynamic field. Also presented is the use of probes for antimicrobial susceptibility testing of bacteria and other infectious agents. The impact of the high cost of this technology on the clinical microbiology laboratory and on the food industry, versus the potential benefits of early diagnosis or recognition of contaminated foodstuffs, is considered. This volume is indispensable to those who work in the field of infectious diseases, including pathologists, microbiologists and infectious disease clinicians. In addition, food microbiologists will also find this volume to be a useful resource.I. INTRODUCTION The age of molecular biology is now becoming the age of molecular medicine. ... cloning vectors, restriction endonucleases, ligase, reverse transcriptase, and the process of DNA-mediated transformation. ... ladder with the adenine and thymine molecules and guanine and cytosine molecules linked together by hydrogen bonds, can be separated into two single strands by heat ( thermalanbsp;...
|Title||:||DNA Probes for Infectious Diseases|
|Author||:||Fred C. Tenover|
|Publisher||:||CRC Press - 1988-11-30|