This volume provides a comprehensive description of the principles and methods used in DNA sequencing. Following a detailed introduction the chapters are: DNA sequencing; Chain terminator sequencing; Primed synthesis methods applied to DNA fragments cloned into phage M13; DNA sequencing by the Maxam-Gilbert chemical procedure; Computer methods for DNA sequencers; Appendices including contractions and special terms, cloning vectors, commercially available restriction endonucleases, and autoradiography.... film cassettes (holders) for autoradiographing sequencing gels in which the film (or gel) can be cut to make a snug fit. ... An electronic flash-gun, masked with an orange filter (e.g. Kodak Wratten number 21 or 22) or alternatively covered withanbsp;...
|Publisher||:||Elsevier - 2000-04-01|