The authors present a comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, 1 vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids and the use of reporter genes, are also described.Introduction Transformation is defined as the transfer of genetic information into a recipient bacterium using naked DNA, without any ... Although not fully understood, chemical methods for the transformation of Escherichia coli probably work byanbsp;...
|Title||:||E. Coli Plasmid Vectors|
|Author||:||Nicola Casali, Andrew Preston|
|Publisher||:||Springer Science & Business Media - 2003-01-01|