Hands-on experts describe in detail the key electron microscopy techniques used for examining cells, tissue, biological macromolecules, molecular structure, and their interactions. Electron Microscopy Methods and Protocols, Second Edition, offers both newcomers and established researchers across experimental biology and medicine wanting to expand their repertoire a gold-standard laboratory manual of cutting-edge electron microscopy techniques-each optimized for reproducibility and robust results-today's gold-standard laboratory manual. New to this edition are sections covering transmission electron microscopy (TEM), and scanning electron microscopy (SEM).Cut thin and/or semi-thin sections after embryos are detected in thick sections by light microscopy. 3.1.5. ... Large montage images can be collected manually and astitched togethera in Photoshop (Adobe) or collected and montaged automatically with analySISTM or similar software. When comparing different embryos within the same mount, it is helpful to make a map using a transmitted light image of theanbsp;...
|Publisher||:||Springer Science & Business Media - 2007|