Enzyme Functionality serves as a conduit for trailblazing research in enzyme engineering-relating current understanding of sequence families, the new notion of enzyme structure classes, and modern methods in protein engineering, design, and directed evolution to accelerate the development of novel enzyme functionalities. This reference gathers the diverse perspectives of nearly 80 scientists from around the globe and surveys all leading rational and random approaches to the artificial evolution of enzymes. Citing more than 1500 notable works, it outlines assays for enzyme activity, stability, and specificity and a wide variety of site-directed, redesign, and evolutionary engineering methods.Functional analysis of the evolutionarily conserved proline 53 residue in Proteus mirabilis glutathione transferase B1a1. FEBS Lett 445:347a350, ... Stereochemical punctuation marks in protein structures: glycine and proline containing helix stop signals. J Mol Biol ... Functional stability of the Ip-subunit of the FoF1-ATPase from Escherichia coli is affected by mutations in three proline residues. Biochimanbsp;...
|Publisher||:||CRC Press - 2003-10-28|