Molecular techniques are emerging as a rapid and powerful approach for microorganism detection. This project reviewed the efficacy of PCR assays for Cryptosporidium, Microcystis, adenovirus and ammonia oxidising bacteria, as well as candidate techniques for DNA extraction and inhibitor removal. The literature review led to an evaluation of DNA extraction kits and reagents for PCR; finalisation of assay formats; the development of PCR controls and Standard Operating Procedures (SOP); analysis of assay robustness using real samples; and completion of an Inter-laboratory trial using the SOPas developed.Working. stocks. 10I¼M AQ1 Reverse primer (5a#39; GCCACGGTGGGGTTTCTAAACTT 3a#39;) 10I¼M AQ2 Forward primer (5a#39; GCCCCAGTGGTCTTACATGCACATC 3a#39;) 10I¼M ADENO Taqman probe (5a#39; FAM- TGCACCAGACCCGGGCTCAGGTACTCCGA-BHQ1 3a#39;) 10mM dNTPs (Invitrogen ) (a ... MgCl2 (Promega) 5x GoTaq PCR Buffer (Promega) 5U/I¼L GoTaq DNA Polymerase (Promega) 100ng/I¼L GP32 (Newanbsp;...
|Title||:||Evaluation and validation of rapid molecular methods for the detection and identification of microorganisms in water - Standard Operating Procedures|
|Author||:||Paul Monis, Nic Reid|
|Publisher||:||IWA Publishing - 2015-06-15|