Herein describes our approach which utilizes cyclic peptide phage display for the evolution of novel cyclic peptide scaffolds that target a given oligonucleotide. Evolved scaffolds are then tested in vitro as discrete entities to assess their binding capabilities. Given that the phage display scaffolds employ a disulfide linker for cyclization, alternative redox stable macrocylic linkers were developed and synthesized. Generated analogues were subsequently assessed for the retention of the desired binding activity.In recent years, it has also been discovered that nucleic acids have capabilities that far exceed their function to store and transmit genetic information. Although the majority of all DNA is found as a B-form double helical structure, DNA hasanbsp;...
|Title||:||Evolution of Cyclic Peptide Scaffolds to Target Nucleic Acids|
|Author||:||Virginia Abigail Burns|
|Publisher||:||ProQuest - 2009|