This volume provides a broad, state-of-the-art coverage of diverse technical topics in gene expression in mammalian cells, including the development of vectors for production of proteins in cultured cells, in transgenic animals, vaccination, and gene therapy; progress in methods for the transfer of genes into mammalian cells and the optimization and monitoring of gene expression; advances in our understanding and manipulation of cellular biochemical pathways that have a quantitative and qualitative impact on mammalian gene expression; and the large-scale production and purification of proteins from cultured cells.This mutant estrogen receptor has been fused to the VP64 transactivation domain as well as to artificial DNA-binding zinc finger domains generating 4-OHT -responsive transcription factors . Depending on different modules ( transactivator, anbsp;...
|Title||:||Gene Transfer and Expression in Mammalian Cells|
|Author||:||Savvas C. Makrides|
|Publisher||:||Gulf Professional Publishing - 2003|