A gold-standard collection of readily reproducible techniques for the molecular and genetic analysis of germ cells in a variety of different reproductive systems. The methods cover sperm and egg activation, motility, fertilization, nuclear development, nuclear cloning, the molecular characterization of specific events, and the imaging of cell structures. Volume 1: Sperm and Oocyte Analysis focuses on sperm cells, oocyte analysis, oocyte maturation, fertilization, and preparation techniques. Volume 2: Molecular Embryo Analysis, Live Imaging, Transgenesis, and Cloning contains methods for molecular egg analysis, live egg imaging, nuclear cloning, oocyte preservation, and nuclear transfer. Comprehensive and cutting-edge, Germ Cell Protocols offers both novice and established researchers a gold-standard collection of hard-to-find methods of high impact research, diverse procedures that are easy-to-follow, well-illustrated, and allow a cross-species transfer of knowledge from lower vertebrates to higher mammalian systems. Use cutting-edge techniques for the molecular and genetic analysis of germ cells Take advantage of cross-species transfer of knowledge from lower to higher vertebrates Perform in vitro maturation and fertilization of human, porcine, and canine oocytes Establish an in vitro spermatogenesis system.Use a Pasteur pipet to transfer fish to 1.5-mL microfuge tubes (10 fish/tube) with as little liquid as possible (10 tubes, ... not fit into one Eppendorf tube, so the mix is divided in half and put into two Eppendorf tubes instead of using a larger tube.
|Title||:||Germ Cell Protocols|
|Publisher||:||Springer Science & Business Media - 2004-01-01|