Guide to Protein Purification, designed to serve the needs of the student, experienced researcher and newcomer to the field, is a comprehensive manual that provides all the up-to-date procedures necessary for purifying, characterizing, and handling proteins and enzymes in one source. Key Features * Detailed procedures newly written for this volume * Extensive practical information * Rationale and strategies for protein and enzyme purification * Personal perspectives on enzyme purification by eminent researchers Among the Topics Covered * General methods for handling proteins and enzymes * Extraction, subcellular fractionation, and solubilization procedures * Comprehensive purification techniques * Specialized purification procedures * Protein characterization * Immunological procedures * Computer analysis of protein structureThe pressure (P\) required to force liquid through a column is a function of mobile phase flow rate (v), sorbent particle ... and interparticle porosity (e) as seen in Eq. (1): PI- PO= 180 vLrtd - e)2/d2Pe2 (1) As noted above, HPLC columns areanbsp;...
|Title||:||Guide to Protein Purification|
|Author||:||Murray P. Deutscher|
|Publisher||:||Gulf Professional Publishing - 1990|