Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study qYour Favorite Geneq in yeast. Key Features * Basic Methods in Yeast Genetics * Physical and genetic mapping * Making and recovering mutants * Cloning and Recombinant DNA Methods * High-efficiency transformation * Preparation of yeast artificial chromosome vectors * Basic Methods of Cell Biology * Immunomicroscopy * Protein targeting assays * Biochemistry of Gene Expression * Vectors for regulated expression * Isolation of labeled and unlabeled DNA, RNA, and proteinEquipment We perform all MS experiments on a nanoflow high-performance liquid chromatography (HPLC) system (Agilent ... The peptide mixtures are loaded onto a 15 cm analytical column (75 mm inner diameter) packed in-house with aanbsp;...
|Title||:||Guide to Yeast Genetics and Molecular Biology|
|Publisher||:||Gulf Professional Publishing - 2004-05-14|