Handbook of Biological Confocal Microscopy

Handbook of Biological Confocal Microscopy

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Once the second edition was safely off to the printer, the 110 larger world of micro-CT and micro-MRI and the smaller world authors breathed a sigh of relief and relaxed, secure in the belief revealed by the scanning and transmission electron microscopes. that they would a€œnever have to do that again. a€ That lasted for 10 To round out the story we even have a chapter on what PowerPoint years. When we ?nally awoke, it seemed that a lot had happened. does to the results, and the annotated bibliography has been In particular, people were trying to use the Handbook as a text- updated and extended. book even though it lacked the practical chapters needed. There As with the previous editions, the editor enjoyed a tremendous had been tremendous progress in lasers and ?ber-optics and in our amount of good will and cooperation from the 124 authors understanding of the mechanisms underlying photobleaching and involved. Both I, and the light microscopy community in general, phototoxicity. It was time for a new book. I contacted a€œthe usual owe them all a great debt of gratitude. On a more personal note, I suspectsa€ and almost all agreed as long as the deadline was still a would like to thank Kathy Lyons and her associates at Springer for year away.TABLE 10.3. Relative Parameters of CCD and EM-CCD Detectors EM-CCD: DV887BV Back Andor E2V CCD 87 512 x 512 16um 8.2 x 8.2 mm 92% ... 1MHz 14/16 bits Masked w/full frame transfer; C-mount Front Andor TI TC285 1000 x 1000 8um 8.0 x 8.0mm agt;65% ... a single photoelectron above the noise level of the CCD-read amplifier has led to the perfection of on-chip electron multiplication (EM).

Title:Handbook of Biological Confocal Microscopy
Author:James Pawley
Publisher:Springer Science & Business Media - 2010-08-04


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