Pseudomonas aeruginosa is a major human pathogen that is naturally resistant to many of the antibiotics currently used to treat microbial infections. It ranks second among all Gram-negative bacteria that pose antibiotic resistance in immunocompromised patients. When 16S rRNA from P. aeruginosa was expressed in the Cunningham lab genetic system, ribosome function was decreased to 46%. Replacement of H9 region from E. coli or the expression of P. aeruginosa S20 protein restored the ribosome function to full amount. We have found that decreased amount of ribosome function in the system containing Pa16S rRNA is due to defect in ribosome assembly. These findings suggest that there is species specific interaction of S20 and helix 9 in P. aeruginosa . Fluorescent binding studies were also performed to validate species-specific interaction.These enhancements have the potential to make peptides better therapeutic agents than small molecules and antibodies.86 87 Despite of ease of synthesis, low cost and long half life, many clinical trials for small molecules have failedanbsp;...
|Title||:||Identification of Drug Targets and Drug Leads in Pseudomonas Aeruginosa|
|Author||:||Ami Khanal Lamichhane|
|Publisher||:||ProQuest - 2008|