This volume provides a timely and useful introduction to the theory and practical application of image analysis in histology. This powerful research technique can be used to detect not only stored products in a cell (immunocytochemistry) but the synthetic machinery and the genes that control it (in situ hybridisation), as well as the specific binding sites that act as receptors for a molecule following its release (in vitro autoradiography). The book provides a good introduction for beginners before looking in greater detail at more advanced material in selected areas. The volume highlights the importance of technique in gathering quantitative information. The book is divided into four sections: introductory material, image acquisition, image processing, and applications. The applications areas include quantitative immunochemistry, quantification of nerves and neurotransmitters and automated grain counting in in situ hybridisation histochemistry.The direction of the lines must be uniform random over 180Ad, to complete the process of creating IUR lines in 3-D space. Vertical sections may ... The cell is sampled only if the nucleus or nucleolus is captured by the dissector. The dissectoranbsp;...
|Title||:||Image Analysis in Histology|
|Author||:||Richard Wootton, David R. Springall, Julia M. Polak|
|Publisher||:||CUP Archive - 1995|