A contribution towards making this increasingly valuable technology accessible to researchers, including the students, post-doctoral scholars, and technicians gathering the knowledge inherent in this integration between analysis and physical isolation/purification methodologies. A step-by-step approach to the methodology for measuring various attributes demonstrated in the particular cells of interest is provided, as is a myriad of resources to fuel the curiosity and answer questions of both new and adept users. This book stems from the editors'experiences managing flow cytometry/cell sorting core facilities for the emerging researchers, in particular in developmental, cellular, and molecular biology.Note: During incubation, use this free time to prepare flow cytometer sample tubes, with 150 ul of cold 2% ... Using an automatic or multichannel pipette, add cold S/W buffer a 100 pil into each well or 1 ml into each Eppendorf tube. 9.
|Title||:||In Living Color|
|Author||:||Rochelle A. Diamond, Susan DeMaggio|
|Publisher||:||Springer Science & Business Media - 2012-12-06|