Investigation of the Plant Cell Cycle in Reference to Human Cancer

Investigation of the Plant Cell Cycle in Reference to Human Cancer

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The cell cycle is an evolutionarily conserved process in all eukaryotes, however, differences are known to exist between kingdoms. Knowledge of the plant cell cycle relies greatly on the knowledge gained from the study of animal and yeast cell cycles. This research, investigating the plant cell cycle, uses a combination of bioinformatic and molecular biology approaches. Analysis of Arabidopsis thaliana, Saccharomyces cerevisiae, and Homo sapiens cell cycle genes shows plants to have considerable similarity to humans. Computational comparisons between the plant expressed gene sequences of Arabidopsis, Oryza sativa, and Zea mays to human and mouse cell cycle genes associated with cancer were used to identify conserved cell cycle genes. The conserved plant sequences were further classified using gene ontologies to distinguish patterns of interest between the plant genomes. It was unexpected to discover that more genes sequences in Arabidopsis are conserved relative to human than are those of larger genomes of rice and maize. Of particular interest was the animal tumor suppressor gene p53. An intensive computational and laboratory analysis for a p53-like tumor suppressor in plants was performed to determine if a p53-like sequence exists in plants. This research found no obvious p53-like homolog in Arabidopsis or maize, however a weak match to a WRKY sequence, a plant transcription factor, was identified. A cell cycle gene conserved in animals and yeast is MAD1 (mitotic arrest deficient 1); this gene is reportedly mutated in lung, lymphoid, breast and prostate cancers. A gene sequence annotated as MAD1 in Arabidopsis, based on nucleotide similarity, is present in the genome but no functional information exists concerning the role of this gene in plants. Analysis of function through complementation of mad1 in yeast, phenotypic growth differences in T-DNA Arabidopsis knockouts, and pollen abnormalities suggests MAD1 of Arabidopsis has a similar function to MAD1 of animals and yeast. This compilation of research further extends the knowledge of the plant cell cycle, and reports on differences and similarities of the cell cycle between kingdoms.Molecular Cloning: A Laboratory Manual. 2 ed. ... Molecular mechanisms of mammalian DNA repair and the DNA damage checkpoints. ... 2001. Mad2 binding to Mad1 and Cdc20, rather than oligomerization, is required for the spindle checkpoint. Embo J 20:6371-82. Sironi, L., M. ... Stechmann, A., and T. Cavalier-Smith.

Title:Investigation of the Plant Cell Cycle in Reference to Human Cancer
Author:Penny May Avoles Kianian
Publisher:ProQuest - 2007


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