This is an unabridged, comb bound version of Ion Channels: Volume 207 of Methods of Enzymology. Techniques for the electrophysiological recording of ion channels in a number of different cell types, including microorganisms, are described in this volume. Key Features * Applications of electrophysiological techniques * Expression in heterologous systems * Reconstitution of lipid bilayers * Purification and characterization of ion channel proteins and genes * Recording of ion channels of intracellular membranes and microorganisms * Data storage and analysisThen the supernatant is carefully removed with a sterile suction pipette, and the pellet is washed with 2 volumes of 70% ethanol. ... To recover the precipitated mRNA the Eppendorf tube is brought to room temperature and centrifuged for 10 min at 12, 000 g, the supernatant is removed, and the opening of the ... Then the mRNA can slowly be resuspended in DEPC-H2O at the desired concentration for use.
|Author||:||Bernardo Rudy, Linda E. Iverson|
|Publisher||:||Gulf Professional Publishing - 1997|