In the post-genomic age, much biomedical research looks at when, where, and at what level genes are expressed. Measuring Gene Expression is an all-in-one introduction to the main methods of measuring gene expression, including RT-PCR, differential display, RNA interference, reporter genes, microarrays, and proteomics, as well as a section on RNA isolation and analysis. There is an overview of each method: its pros and cons, sample preparation, sources of error, and data interpretation.Dual hybridization probes This is a relative of the TaqMan system, and follows a similar procedure (Wittwer etal., 1997). ... Chemical blocking of the 3a² end of this quencher probe is undertaken to prevent it from being used as a PCR primer. ... this case can be devoid of 5a²a3a² exonuclease activity, since the probes do not need to be degraded for the system to work, though it does not matter if they are.
|Title||:||Measuring Gene Expression|
|Publisher||:||Garland Science - 2005-01-28|