To test this hypothesis, rat BMMSCs were exposed to physiologically relevant magnitudes and frequencies of a Mechanical Panel, which consisted of cyclic stretch, cyclic pressure, and shear stress, each applied in parallel to subcultures of BMMSCs. Quantitative changes in morphology, proliferation, and gene and protein expression were assessed to determine the differential effect of each stimulus in a dose- and frequency-dependent manner. Next, we investigated the importance of the duration of applied stimulation to BMMSC differentiation as well as tissue commitment (i.e., cell plasticity) following mechanical stimulation.TaqManAr gene expression assays are built on 5a#39; nuclease chemistry, with each assay containing two unlabeled PCR ... Working within these limits, primers were chosen to represent almost all possible phenotypic outcomes that could beanbsp;...
|Title||:||Mechanobiology of Stem Cells: Implications for Vascular Tissue Engineering|
|Publisher||:||ProQuest - 2007|