Providing information on the main approaches for the analysis of metabolites, this textbook: Covers basic methodologies in sample preparation and separation techniques, as well as the most recent techniques of mass spectrometry. Differentiates between primary and secondary metabolites. Includes four chapters discussing successful metabolome studies of different organisms. Highlights the analytical challenges of studying metabolites. Illustrates applications of metabolome analysis through the use of case studies.There are three solid shear methods that are relevant for metabolome analysis: manual grinding, ball mill, and Ultra-Turrax. ... Cell disruption in ball mills is regarded as an optimized alternative for the classic mortar and pestle. ... more accessible and easy to handle, and recent approaches on metabolic footprinting analysis (Allen et al., 2003; Villas-Boas et al., 2005b, 2006) have demonstrated how usefulanbsp;...
|Author||:||Silas G. Villas-Boas, Jens Nielsen, Jorn Smedsgaard, Michael A. E. Hansen, Ute Roessner-Tunali|
|Publisher||:||John Wiley & Sons - 2007-02-16|