This new volume of Methods in Cell Biology looks at methods for analyzing of golgi complex function. Chapters cover such topics as in vitro reconstitution systems, fluorescence-based analysis of trafficking in mammalian cells and high content screening. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge materialNext, put the resin block into the holder of the ultramicrotome and examine it under a stereomicroscope. ... 3D. reconstruction. In our experience, identifying the structure of interest under the electron microscope is facilitated by ... other GFP-labeled organelles that were observed in vivo together with the structure of interest, a the cell nucleus, a particular features of cell shape and/or cell protrusions.
|Title||:||Methods for analysis of Golgi complex function|
|Publisher||:||Elsevier - 2013-11-26|