This unique volume presents leading-edge microfluidics methods used to handle, manipulate, and analyze cells, particles, and biological components (e.g., proteins and DNA) for microdiagnostics. The authors offer clear and detailed guidance on microfabrication techniques utilized to create microfluidic devices and on-chip flow control and mixing Microsystems, protein and DNA handling devices for electrophoretic and isoelectric separations in microchromatography columns, microfluidic manipulations of droplets via electrowetting and particles via dielectrophoresis for separations and chemical reactions, integrated optical characterization of microfluidic devices, controlling chemical gradients within devices, microimmunoassay diagnostics, multiphase microfluidics used in droplet formation for controlled chemical reactions, particle separation and analysis in Micro-FACS systems, flow characterization techniques in microfluidic devices and patterning and utilizing cytoskeletal filaments and cellular transport protein within microstructures.Use in-line luer-lock filter to yield filtered solutions. ac Aliquot solution droplets from the syringe and filter system directly into the well, or filter out the solution first into an Eppendorf tube, then pipette solution into the well. If using droplets directlyanbsp;...
|Title||:||Methods in Bioengineering|
|Author||:||Jeffrey D. Zahn|
|Publisher||:||Artech House - 2014-05-14|