Despite being recognized and fought against over countless centuries, human viral pathogens continue to cause major public health problems worldwideakilling millions of people and costing billions of dollars in medical care and lost productivity each year. With contributions from specialists in their respective areas of viral pathogen research, Molecular Detection of Human Viral Pathogens provides a reliable reference on molecular detection and identification of major human viral pathogens. Each chapter briefly reviews the classification, epidemiology, clinical features, and diagnosis of one related viral pathogen or a group of them. The clinical sample collection and preparation procedures are outlined, and a selection of representative stepwise molecular detection protocols is covered. The chapters conclude with a discussion on further research requirements relating to improved diagnosis. With its judicious selection of streamlined, ready-to-use protocols for major human viral pathogensaincluding commercial kitsaMolecular Detection of Human Viral Pathogens is an indispensable tool for medical, veterinary, and industrial laboratory scientists involved in virus determination.The same type of fluorescent thermocycler used for TaqMan is also necessary for this assay. ... work areas; one ac dedicated to preamplification RNA work (RNA extraction and RT-PCR setup) and the other for postamplification analysis ( agarose gel electrophoresis). ... Open and close tubes quickly and avoid touching ac any inside or lip/mouth portions (do not attempt to open tubes with single hand).
|Title||:||Molecular Detection of Human Viral Pathogens|
|Publisher||:||CRC Press - 2012-10-05|