MutL is an essential component of the Escherichia coli mismatch repair (MMR) pathway and has been shown to interact with nearly every protein involved in the MMR pathway including MutS, MutH and UvrD.The proteins were transferred to nitrocellulose by applying an electric potential of 14.5 V/cm across the gel for 60 min at 4oC. The nitrocellulose filter paper was blocked with 2 % (w/v) powdered milk (Carnation) in 25 ml TTBS (10 mM Tris HCl anbsp;...
|Title||:||MutL Involvement in Two DNA Repair Pathways: Methyl-directed Mismatch Repair and Very Short Patch Repair|
|Author||:||Adam Brian Robertson|
|Publisher||:||ProQuest - 2007|