An indispensable collection of novel techniques that have proven most useful for studying the physiological properties of p53 both in vitro and in vivo. The techniques provide proven solutions to problems in studying the purification, target identification, gene expression, quantitation, interaction, signaling, transactivation, and transrepression of p53. The methods are also useful for delineating the functions of other proteins that may act as tumor or growth suppressors. Each technique includes step-by-step instructions, troubleshooting notes, a theoretical review, and discussion of associated problems that might arise during the course of investigation.For every plate to be transfected, take out and label appropriately two 1.5-mL Eppendorf tubes, one for the DNA and one for the HEBS/PO4 solution. 6. In a 50- mL Falcon ... Using a pipet-aid, set up a abubbling apparatus.a Clamp the pipet- aidanbsp;...
|Author||:||Sumitra Deb, Swati Palit Deb|
|Publisher||:||Springer Science & Business Media - 2003-01-01|