A thoroughly updated version of the successful first edition with a new chapter on Real-Time PCR, more prokaryotic applications, and more detail in the complex mutagenesis sections. Information on PCR applications in genomics and proteomics have been expanded and integrated throughout the text. There is also advice on available products and specific pointers to the most appropriate methods. As with the first edition, this will be an ideal practical introduction and invaluable guide to PCR and its applications.Nonetheless, it is a highly powerful strategy that works efficiently and one which you could adapt to many PCR cloning and mutagenesis ... The changes will therefore be introduced into both strands of the newly synthesized plasmid DNA.
|Author||:||Mike McPherson, Simon Møller|
|Publisher||:||Taylor & Francis - 2007-01-24|