PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.Bacteria that contain clones that are positive for the correctly ligated DNA insert will contain a PCR DNA fragment that is ... Once positive colonies have been identified, plasmid DNA is prepared by the alkaline lysis or other DNA mini-prepanbsp;...
|Title||:||PCR Cloning Protocols|
|Author||:||Bing-Yuan Chen, Harry W. Janes|
|Publisher||:||Springer Science & Business Media - 2002|