PCR for Clinical Microbiology

PCR for Clinical Microbiology

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Not another textbook, but a valuable tool for doctors and microbiologists wanting to know how to set up a PCR diagnostic microbiology laboratory according to current regulatory standards and perform assays supplied with patient clinical diagnostic criteria and easy to follow protocols. Whether laboratories are using commercial kits or in-house methods developed in their own laboratories or adopted from published methods, all clinical microbiology laboratories need to be able to understand, critically evaluate, perform and interpret these tests according to rigorous and clinically appropriate standards and international guidelines. The cost and effort of development and evaluation of in-house tests is considerable and many laboratories do not have the resources to do so. This compendium is a vehicle to improve and maintain the clinical relevance and high quality of diagnostic PCR. It is a unique collection of; guidelines for PCR laboratory set up and quality control, test selection criteria, methods and detailed step by step protocols for a diagnostic assays in the field of molecular microbiology. The structure of the book provides the PCR fundamentals and describes the clinical aspects and diagnosis of infectious disease. This is followed by protocols divided into; bacteria, virus, fungi and parasites, and susceptibility screens. The inclusion of medical criteria and interpretation adds value to the compendium and benefits clinicians, scientists, researchers and students of clinical diagnostic microbiologyThe method does not detect the other three known species of Brucella, and if these are suspected, conventional Brucella PCR with gel based detection should be employed [2]. The PCR ... A genus specific TAQman PCR is under development but not yet validated. ... The method is based upon the work of Redkar et al. [1].

Title:PCR for Clinical Microbiology
Author:Ian W.J. Carter, Margret Schuller, Gregory S. James, Theo P. Sloots, Catriona L. Halliday
Publisher:Springer Science & Business Media - 2010-07-03


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