PCR in Bioanalysis offers powerful PCR-based protocols and assays in actual use or potential use in clinical medicine and commercial biology. The main focus of the book is on the commercial applications of PCR, as opposed to basic research uses. Topics covered include the measurement of hormone levels using PCR, transcription factor isolation, detection of viruses using PCR, detection of tumor contamination of stem cells, evaluation of grafts for tumor cells, and more.Essentially, the target PCR product is cloned into a suitable plasmid vector, such as pBluescript, which has promoters for the T7 and the T3 RNA ... This is important, as it ensures that the eventual clone will not contain sequence errors.
|Title||:||PCR in Bioanalysis|
|Author||:||Stephen J. Meltzer|
|Publisher||:||Springer Science & Business Media - 1998-01-01|