This second edition of a much-praised and widely used manual has been entirely revised and updated. Each technique is presented with extensive background information, advice, and troubleshooting. All current applications of PCR are covered, in protocols that have the hallmark reliability of the previous edition.DNA polymerases with a 3a#39; exonuclease (proofreading) activity, such as Pfu, Vent , and DeepVent DNA polymerases, however, ... working practices and an effective decontamination protocol can control the problem of carryover contamination.
|Author||:||Carl W. Dieffenbach, Gabriela S. Dveksler|
|Publisher||:||Cold Spring Harbor Laboratory Pr - 2003|