Drawing on the highly successful first edition, this newly-revised second edition covers the many advances made in PCR technology since the first book, which has been used in more than 10, 000 laboratories worldwide. As PCR technology has advanced significantly, its use has grown in the clinical laboratory of physician/researchers, the scope of this book is greatly expanded to enable researchers at all levels to easily reproduce and adapt PCR experiments to their own specific requirements. The methods selected represent worked examples from many fields that can be reproduced and adapted for use within the reader's laboratory. The authors have provided both a primer to allow the reader to gain basic experience of different PCR techniques, as well as in-depth insight into a variety of the more complex applications of PCR. This book will be essential for the labs of all biochemists, molecular biologists, geneticists and researchers utilizing the PCR technique in their work. 71 chapters of the most important PCR methodologies for your lab Includes the newest and most up-to-date collection for using PCR in a wide range of applications Provides an extensive range of versatile, expedient, and readily applicable PCR protocols Protocols are suitable for both novice and experienced researchers Notes section in each chapter provides tips, alternative suggestions, and other enhancements of the protocols.DNA modification can occur during PCR because the primers are incorporated into the ends of the PCR product. ... can recombine to form a circle, and if this circle constitutes a selectable plasmid, E. coli can be readily transformed by the linear PCR products. Recombination PCR has almost no steps apart from PCR amplification and transformation of E. coli, and this method works well in Rec A minus E.
|Author||:||John M. S. Bartlett, David Stirling|
|Publisher||:||Springer Science & Business Media - 2003-01-01|