PCR Strategies expands and updates the landmark volume PCR Protocols. It is a companion laboratory manual that provides a completely new set of up-to-date strategies and protocols for getting the most from PCR. The editors have organized the book into four sections, focusing on principles, analyses, research applications, and alternative strategies for a wide variety of basic and clinical needs. If you own PCR Protocols, you will want PCR Strategies. If you don't own PCR Protocols, you will want to buy both! Key Features * Concepts explained * Methods detailed * Trouble-shooting emphasized * Novel applications highlighted * Major sections * Key concepts for PCR * Analysis of PCR products * Research applications * Alternative amplification strategiesshould not be placed at the extreme 3a#39; end of the oligonucleotide because primer extension will be inhibited (Kwok et al., 1990). ... This clever idea was proposed but not actually used for PCR mutagenesis (Kadowaki et al., 1989), so it is not possible to evaluate how well it works in practice. Another method for a embeddinga a mutation inside a PCR product uses ainversea PCR to amplify an entire plasmidanbsp;...
|Author||:||Michael A. Innis, David H. Gelfand, John J. Sninsky|
|Publisher||:||Academic Press - 1995-07-06|