Phage-display technology has begun to make critical contributions to the study of molecular recognition. DNA sequences are cloned into phage, which then present on their surface the proteins encoded by the DNA. Individual phage are rescued through interaction of the displayed protein with a ligand, and the specific phage is amplified by infection of bacteria.A Laboratory Manual Carlos F. Barbas ... In addition, because the radioisotope used is 33P, the gel must be fixed and dried before exposure. ... caps for PCR ( VWR, Cat. # 20170-004 or similar), autoclaved (in a clean-steam autoclave, see Appendix 3) and then oven-baked (at 105AdC for 4 hours) PCR machine Caution: 33Panbsp;...
|Author||:||Carlos F. Barbas|