In Plant Cell Culture Protocols, Robert Hall and a panel of expert researchers present a comprehensive collection of the most frequently used and broadly applicable techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods cover culture initiation, maintenance, manipulation, application, and long-term storage, with emphasis on techniques for genetic modification and micropropagation. Many of these protocols are currently used in major projects designed to produce improved varieties of important crop plants. In addition, a number of specialized protocols have been included to illustrate the diversity of the techniques available and their widespread applicability. Plant Cell Culture Protocols is aimed at scientists involved in all aspects of plant biotechnological research, as well as those working in other areas of agriculture and horticulture who are interested in expanding their technical repertoire to include in vitro methodology. Its state-of-the-art techniques are certain to make the book today's reference of choice, an indispensable tool in the development of new transgenic plants and full-scale commercial applications.Transformation. via. Protoplast. Electroporation. George W. Bates 1. Introduction In electroporation, cells are ... Electroporation is effective on virtually any type of cell, and is now the method of choice for the genetic transformation of bacteria and certain animal cell lines. ... Tissue electroporation does not work in all cases, and the parameters for successful plant tissue electroporation are not yet clear.
|Title||:||Plant Cell Culture Protocols|
|Author||:||Robert D. Hall|
|Publisher||:||Springer Science & Business Media - 1999-01-01|