This volume successfully and clearly examines how biophysical approaches can be used to study complex systems of reversibly interacting proteins. It deals with the methodology behind the research and shows how to synergistically incorporate several methodologies for use. Each chapter treats and introduces the reader to different biological systems, includes a brief summary of the physical principles, and mentions practical requirements.... fluorescence intensity distribution analysis (2D-FIDA) (Kask et al., 2000) and the dual-color photon counting histogram (dual-color PCH) (Chen et al., 2005). ... Cy5), (2) species associated to s70, the initiation factor (labeled with a single yellow dye, Tetramethylrhodamine), ... Mostofthem are relatively recent, and have not been applied to solve problems of biological interest, exceptfor PCH and FIDA.
|Publisher||:||Springer Science & Business Media - 2007-03-20|