This volume and its companions (Volumes 255, 256, 257, and the forthcoming 329) cover all biochemical and biological assays currently in use for analyzing the role of small GTPases in these aspects of cell biology at the molecular level. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.The beads are collected by brief centrifugation (2000 rpm, 1 min at 4 in a Beckman bench-top centrifuge). They are washed six times with 12 ml wash buffer (50 mM Tris, pH 7.5, 0.5% Triton X-100, 150 mM NaCl, 5 mM MgCl2 , 1mM DTT, 1 g/ml aprotinin ... This is the most common problem when the assay is not working well.
|Title||:||Regulators and Effectors of Small GTPases, Part D: Rho Family|
|Publisher||:||Academic Press - 2000-10-11|