A panel of highly regarded molecular biologists and clinical researchers describe in detail their most novel, useful, and interesting RT-PCR applications. Here the newcomer will find readily reproducible protocols for highly sensitive detection and quantification of gene expression, the in situ localization of gene expression in tissue, and the cloning of genes, as well as for analyzing T-cell clones and the differential expression of genes. For the expert seeking to extend the usefulness of RT-PCR, there are user-friendly applications that complement the latest technological advances, including laser-capture microdissection (LCM), real-time and quantitative PCR, microarray technology, cDNA cloning, and antibody engineering. Study disease pathogenesis with RT-PCR to design new therapeutic strategies Expand RT-PCR with antibody engineering, real-time PCR, and microarray technology.After purifying the linearized plasmid from the small excised fragment by gel filtration chromatography, the sticky ends of ... the regular sense primer sequence to the 5a#39; end of the internal primer, PCR with this composite primer will generate a ... The first requirement is to select a primer pair that works well in regular RT- PCR.
|Publisher||:||Springer Science & Business Media - 2002-01-01|