SARS- and Other Coronaviruses

SARS- and Other Coronaviruses

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In 2003, the word qcoronavirusq spread across the globe, somewhat further than the virus that sparked the panic. In SARS- and Other Coronaviruses: Laboratory Protocols, expert researchers examine these devastating viruses through detailed laboratory protocols. Chapters deal with such subjects as detection and discovery of coronaviruses by nucleic acid and antibody-based approaches, virus isolation, propagation and titration, virus purification, structure analysis by electron cryomicroscopy, expression and crystallization of viral proteins, raising antibodies against viral proteins, manipulation of the coronavirus genome, and descriptions of how to investigate aspects of the cell surface for coronavirus receptors. Composed in the highly successful Methods in Molecular BiologyTM series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, SARS- and Other Coronaviruses: Laboratory Protocols serves as an ideal guide for all virologists and especially for those working with coronaviruses.A vibrating engraver (Fisher Scientific) or drill (Dremel) is used to prepare holes in the egg shells. Prior to ... A suitable egg incubator is needed; these are available from a variety of commercial sources. ... Methods Embryonated chicken and turkey eggs are extensively utilized for isolation and propagation of IBV and TCoV, anbsp;...

Title:SARS- and Other Coronaviruses
Author:Dave Cavanagh
Publisher:Springer Science & Business Media - 2008-09-02


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