Many investigations into the structure and function of cells and tissues require the isolation of a particular membrane or subcellular component (organelle). This book covers all the necessary aspects, from breaking up the cells (homogenization), via a variety of separation techniques (the isolation and fractionation chapters), to characterization of the separated organelles. - ;Many investigations into the structure and function of cells and tissues require the isolation of a particular membrane or subcellular component (organelle). This book covers all the necessary aspects, from the homogenization of cells via a variety of separation techniques, to the isolation and characterization of peroxisomes and other organelles. Practical guidance on basic separation techniques needed in all cell biology laboratories is given. These techniques are presented in the context of a number of related topics such as the labelling of ligands, the kinetics of their internalization and analysis of data, and the electron microscopy of organelles. -... approach to differential pelleting is not to centrifuge a uniform suspension of particles but to layer the particles over a denser liquid. ... but if, instead of a uniform column of liquid we layer the particles over a density gradient, then we do get an orderly separation. ... Damage to organelles during separation is a much greater problem in isopycnic banding than in rate-zonal centrifugation for three reasons.
|Title||:||Subcellular Fractionation : A Practical Approach|
|Author||:||J. M. Graham, D. Rickwood|
|Publisher||:||Oxford University Press, USA - 1997-01-30|