Techniques in Protein Chemistry III compiles papers presented at the Fifth Protein Society Symposium in Baltimore on June 22-26, 1991. This book discusses the protein and peptide recovery from PVDF membranes; high-sensitivity peptide mapping utilizing reversed-phase microbore and microcolumn liquid chromatography; and capillary electrophoresis for preparation of peptides and direct determination of amino acids. The TFMSA/TFA cleavage in t-Boc peptide synthesis; applications of automatic PTC amino acid analysis; and identification of O-glycosylation sites with a gas phase sequencer are also elaborated. This text likewise covers the conformational stability of the molten globule of cytochrome c and role of aqueous solvation in protein folding. This publication is useful to students and researchers interested in methods and research approaches on protein chemistry.Acknowledgements This work was supported by the University of Wisconsin Biotechnology Center, NIH grants 1 S10 RR02971, S10 RR03427 and ... Protein/ Peptide Sequencer Users Manual Model 477A (1989) Applied Biosystems Inc. 7.
|Title||:||Techniques in Protein Chemistry III|
|Author||:||Ruth Hogue Angeletti|
|Publisher||:||Academic Press - 2014-06-28|