This second volume of the Metabolic Pathway Engineering Handbook delves into evolutionary tools and gene expression tools for metabolic pathway engineering. It covers applications of emerging technologies including recent research genome-wide technologies, DNA and phenotypic microarrays, and proteomics tools for experimentally determining flux through pathways. This volume also looks at emerging applications for producing fine chemicals, drugs, and alternative fuels. Christine Smolke, who recently developed a novel way to churn out large quantities of drugs from genetically modified breweras yeast, is regarded as one of the most brilliant new minds in biomedical engineering. In this handbook, she brings together pioneering scientists from dozens of disciplines to provide a complete record of accomplishment in metabolic pathway engineering. With a wealth of cutting edge research and analysis, this work also serves as an invaluable resource for those seeking to add their own contributions. Organized by topic, this 3000 page reference is available as two volumes that can be purchased individually or as a set.At this time, one can also streak onto ampicillin or tetracycline plates (depending on the Red plasmid used), to identify ... (5 I¼l of overnight culture in a 100 I¼l PCR works well), or a use a target contained within a nonreplicative plasmid.
|Title||:||The Metabolic Pathway Engineering Handbook|
|Publisher||:||CRC Press - 2009-07-28|